In this paper, different parts of peanuts as raw material, the concentration of ethanol, liquid ratio, extraction temperature, extraction time the impact of four factors that the uniform design experiment, in order to optimize the return from the peanut extract luteolin extraction process efficient. Luteolin a standard for the control, by ultraviolet spectrophotometry of luteolin extraction were determined. The results showed that extraction of peanuts in return luteolin optimization conditions were as follows: 50% ethanol concentration, liquid ratio of 1:30, extraction temperature of 90 ℃, extraction back to 2.5h, peanut Luteolin the highest rate of extraction 1.02%, to optimize the results of this experiment can be used to extract the next phase HPLC, and used to produce