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tail PCR 电泳后没有条带的原因是什么
tail PCR 电泳后没有条带的原因是什么
2025-03-16 16:52:13
推荐回答（1个）
回答1：
模板降解，引物特异性差，试剂失活，扩增条件不适，退火温度不适，建议重新提取模板，验证引物特异性，并采用高特有试剂盒扩增（高保真酶扩增），尝试降落PCR，摸索退火温度（引用，仅供参考）
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