PCR产物的纯化中所用的PB液的主要成分及其各种成分的主要作用

2024-12-22 21:58:30
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回答1:

PB=Sodium phosphate buffer.

根据厂家,浓度可以不同:
0.1 M

NaH2PO4 * H2O 3.1 g
Na2HPO4 (anhydrous) 10.9 g

Distilled water to make a total volume of 1 liter. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 month in 4°C.

0.2 M
NaH2PO4 * 2 H2O 5.93 g
Na2HPO4 (anhydrous) 23.0 g