Take the fresh Chinese yam as raw material, studies in the Chinese yam the polyphenoloxidase (PPO) most suitable pH value, the optimum temperature, the most suitable substrate concentration and the chemical additive influence, designed has protected the color plan differently, established the Chinese yam to protect the color condition best. Uses the spectrophotometric method to study the pH value, the temperature to the enzyme activity influence; Compared with protects the color fluid differently to the enzyme vigor influence; The result indicated that take the catechol as the substrate, this enzyme most is suitable affects pH is 5.8, most suitable function temperature for 20℃, Km=1.82mol/L, Vmax=1.11 (DELTA OD/min). This article simultaneously inspected the inhibitor to the Chinese yam polyphenoloxidase active influence, thus formulates protects the color measure best. key word: Chinese yam; Polyphenoloxidase; Dynamics characteristic; Turning brown; Protects the color
新鲜な山芋を原料にして、山芋の中で多いフェノールの酸化酵素(PPO)の最も気分が良いpH値を研究します、最も気分が良い温度、最も気分が良い底の物の浓度と添加剤の影响、色の方案をかばうことと设计して、山芋の最も良いのが色の条件をかばうことを确立します。光度法を全て分けてpH値、温度の酵素の活性に対する影响を研究することを采用します;比较的に色の液体の酵素の活力に対する影响をかばうことと;隣のベンゼンの2フェノールを底の物にして、この酵素の最も気分が良い作用pH 5.8のため、最も気分が良い作用の温度は20℃で、Km=1.82mol/L,Vmax=1.11(Δ OD/min)と结局表明しています。本文は同时に抑制剤の山芋の多いフェノールの酸化酵素の活性に対する影响を考察して、それによって最も良いことを制定して色の措置をかばいます。
キーワード:山芋;多いフェノールの酸化酵素;动力学の特性;褐変します;色をかばいます
Take the fresh Chinese yam
as raw material, studies in
the Chinese yam the
polyphenoloxidase (PPO) most
suitable pH value, the optimum
temperature, the most suitable
substrate concentration and the
chemical additive influence,
designed has protected the color
plan differently, established
the Chinese yam to protect the
color condition best. Uses the
spectrophotometric method to
study the pH value, the temperature
to the enzyme activity influence;
Compared with protects the color
fluid differently to the enzyme
vigor influence; The result
indicated that take the catechol
as the substrate, this enzyme
most is suitable affects pH is 5.8,
most suitable function temperature
for 20℃, Km=1.82mol/L, Vmax=1.11 (DELTA OD/min).
This article simultaneously
inspected the inhibitor to the Chinese
yam polyphenoloxidase active influence,
thus formulates protects the color measure
best. key word: Chinese yam; Polyphenoloxidase;
Dynamics characteristic; Turning
brown; Protects the color
好的 恩呢
Take the fresh Chinese yam as raw material, studies in the Chinese yam the polyphenoloxidase (PPO) most suitable pH value, the optimum temperature, the most suitable substrate concentration and the chemical additive influence, designed has protected the color plan differently, established the Chinese yam to protect the color condition best. Uses the spectrophotometric method to study the pH value, the temperature to the enzyme activity influence; Compared with protects the color fluid differently to the enzyme vigor influence; The result indicated that take the catechol as the substrate, this enzyme most is suitable affects pH is 5.8, most suitable function temperature for 20℃, Km=1.82mol/L, Vmax=1.11 (DELTA OD/min). This article simultaneously inspected the inhibitor to the Chinese yam polyphenoloxidase active influence, thus formulates protects the color measure best. key word: Chinese yam; Polyphenoloxidase; Dynamics characteristic; Turning brown; Protects the color