用DNAMan设计的引物,其中有一条为GAGAGGAAGGAGAAGAGAATGG,只含3种碱基,没有C,不知道这种引物可用吗?

2024-12-19 16:50:45
推荐回答(2个)
回答1:

挺好的,帮你测试了下
Primer 1
--------
Primer: GAGAGGAAGGAGAAGAGAATGG
Length: 22
%GC: 50.0
Tm: 58.5 deg C
1 礸 of primer: 143.378 pMole ends
Self-annealing: maximum score = 4
GAGAGGAAGGAGAAGAGAATGG
||
GGTAAGAGAAGAGGAAGGAGAG
3' end score = 1
GAGAGGAAGGAGAAGAGAATGG
:
GGTAAGAGAAGAGGAAGGAGAG

回答2:

安全搞不懂