挺好的,帮你测试了下
Primer 1
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Primer: GAGAGGAAGGAGAAGAGAATGG
Length: 22
%GC: 50.0
Tm: 58.5 deg C
1 礸 of primer: 143.378 pMole ends
Self-annealing: maximum score = 4
GAGAGGAAGGAGAAGAGAATGG
||
GGTAAGAGAAGAGGAAGGAGAG
3' end score = 1
GAGAGGAAGGAGAAGAGAATGG
:
GGTAAGAGAAGAGGAAGGAGAG
安全搞不懂